Skip to main content
. 2009 Mar 30;284(23):15857–15866. doi: 10.1074/jbc.M808986200

FIGURE 1.

FIGURE 1.

Wnt-5a induces clustering of the scaffold protein PSD-95 in mature hippocampal neurons. A, representative images of neurite immunofluorescence of PSD-95 (green) and fluorescence labeling of processes by phalloidin (blue) from control medium (a), Wnt-5a (b), or Wnt-5a plus sFRP (c) after 1 h of treatment. d, quantification of the number of PSD-95 clusters/100 μm of neurite (n = 5). B, time course of the effect of Wnt-5a on PSD-95 analyzed by quantification of PSD-95 clusters/100 μm of neurite (n = 3). C, Western blot of total PSD-95 levels of hippocampal neurons exposed to Wnt-5a (n = 3). D, representative images of dendritic spines of enhanced GFP-expressing neurons (green) (b and e), stained against PSD-95 (red) (a and d), and dendritic spines of enhanced GFP-expressing neurons stained against PSD-95 (c and f) of hippocampal neurons exposed to Wnt-5a (d–f) or control conditioned medium (a–c) for 2 h. g, quantification of PSD-95-positive dendritic spines/10 μm. Scale bar, 5 μm. *, p < 0.01.