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. 2009 Mar 30;284(23):15857–15866. doi: 10.1074/jbc.M808986200

FIGURE 3.

FIGURE 3.

A diffuse pool of PSD-95 is recruited to the membrane pool by Wnt-5a ligand. Hippocampal neurons were incubated in the presence or absence of Wnt-5a for 2 h, and the distribution of PSD-95 in the particulate fraction (A) and cytoplasmic fraction (B) was evaluated by Western blot. As control, hippocampal neurons were incubated with DMSO (vehicle), 2-bromopalmitate (2BP), and palmitate (Palm) for 4 h, and a subcellular fractionation was performed. *, the cross-reactivity of monoclonal PSD-95 antibody with bands of slightly slower migration that correspond to other MAGUK proteins (46). The mobility of the protein PSD-95 to the particulate fraction under the activity of the Wnt-5a ligand was quantified (C). The movement of the protein PSD-95 to the particulate fraction was controlled by treatment with 2-bromopalmitate and palmitate. Protein PSD-95 remains in the cytosolic fraction under the activity of the ligand is shown in D, also using the palmitate and 2-bromopalmitate controls. Western blots were performed against the protein PSD-95, and the 95 kDa band was quantified (n = 3). *, p < 0.05.