Wnt-5a activates noncanonical Wnt pathways on 14-DIV hippocampal neuron cultures. A, quantification of Western blot of β-catenin in hippocampal neurons exposed to Wnt-5a and Wnt-7a in a temporal course (n = 3). B, representative Western blot of β-catenin in neurons stimulated with Wnt-5a, Wnt-7a, or both, co-incubated with sFRP for 1 h (n = 4). C, representative Western blot of total and phosphorylated CAMKII in neurons treated for different times with Wnt-5a ligand (n = 3). D, representative Western blot of total and phosphorylated JNK in neurons stimulated with Wnt-5a for different times. In the graphs, JNK2/3 = 55 kDa and JNK1 = 46 kDa. (n = 5). *, p < 0.05.