FIGURE 7.

The CXCR4 promoter was activated by Notch-RBP-J signaling. A, reporter assay. DC2.4, HEK293, and HeLa cells were transfected with pGL-CXCR4 and increasing amounts (0, 50, 100, and 150 ng) of pEFBOS-NIC (NIC). Cells were collected 48 h after the transfection, and the luciferase activity in lysates was assayed. B, different truncates of the mouse CXCR4 promoter were fused with the basic promoter and luciferase gene, and were used for reporter assay as in A. The amounts of pEFBOS-NIC added were 0, 100, and 150 ng. Bars represent means ± S.D. (n = 5).