Table 1.
Percentage of total fishing events in specified dwell time ranges
| Components in chambera |
Fishing time, ms |
Total events |
Events < 5.61 msb |
Events ≥ 5.61, < 75.22 msc |
Events ≥ 75.22 ms |
|---|---|---|---|---|---|
| DNA | 500 | 609 | 98.01% | 1.99% | 0% |
| DNA, KF | 500 | 853 | 61.14% | 38.04% | 0.82% |
| DNA, KF, dGTP | 500 | 536 | 14.99% | 9.51% | 75.5% |
| DNA, KF, dGTP | 100 | 537 | 14.63% | 9.01% | 76.36% |
| DNA, KF, dGTP | 50 | 269 | 39.42% | 11.3% | 49.28% |
| DNA, KF, dGTP | 10 | 1052 | 82.36% | 7.15% | 10.49% |
| DNA, KF, dGTP | 7.5 | 670 | 91.55% | 5.54% | 2.92% |
| DNA, KF, dGTP | 5 | 668 | 97.96% | 0.73% | 1.31% |
a
The concentration of each component in this experiment, when present as indicated, was: 1 µM DNA, 2 µM KF, and 400 µM dGTP.
b
This upper dwell time limit for unbound DNA events was calculated as the time below which 98% of events occurred when the DNA hairpin was in the cis chamber absent KF and dGTP (Figure 5a).
c
An upper dwell time limit (75.22 ms) for binary complex events was defined as the time below which 98% of the events that exceed the first boundary of 5.61 ms occur, when both DNA and KF were present (Figure 5b).