Table 2. Effect of Ku-0063794 on seven lipid kinases.
Results are presented as percentage of lipid kinase activity compared with control incubations in which Ku-0063794 was omitted. Lipid kinases were assayed as described in the Materials and methods section, in the absence or presence of the indicated concentration of Ku-0063794. The results are an average of a triplicate determinations±S.D.
| Percentage of activity remaining | ||
|---|---|---|
| Kinase | Ku-0063794 (1 μM) | Ku-0063794 (10 μM) |
| PI3Ks | ||
| Class I | ||
| PI3Kα | 97±2 | 95±3 |
| PI3Kβ | 95±9 | 84±22 |
| Class II | ||
| PI3KC2β | 126±13 | 82±12 |
| Class III | ||
| VPS34 | 101±4 | 93±1 |
| Other lipid kinases | ||
| Choline kinase | 102±1 | 102±1 |
| SPHK1 | 108±3 | 99±7 |
| SPHK2 | 119±2 | 116±3 |