Skip to main content
. 2009 Jun 26;421(Pt 2):273–282. doi: 10.1042/BJ20090175

Figure 3. Gel-shift assay of binding of CRx to intact RAP.

Figure 3

Binding titration of CR56, CR345 and CR456 to RAP by non-denaturing gel-shift assay. The Figure show the migration of 2.5 μg of RAP either alone (RAP) or incubated with the indicated molar ratios of CR by 8% non-denaturing PAGE. (A) RAP incubated with 1–7 molar ratios of CR56. (B) RAP incubated with 1–8 molar ratios of CR345. (C) RAP incubated with 1–8 molar ratios of CR456. The migration of 7 μg of the indicated CR alone is shown in the far-left lane of each respective gel.