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. 2009 Jun 26;421(Pt 2):273–282. doi: 10.1042/BJ20090175

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Titration of D1 into 1.8 μM CR56; (B) titration of D2 into 4.6 μM CR56; (C) titration of D3 into 1.8 μM CR56; (D) titration of CR56 into 3 μM RAP. The upper part of each panel gives the raw ITC data for the titration prior to correction for dilution of titrant, and the lower portion gives the best fit of the data to a single site model (A–C) or a two-site model (D).