Table 4. Analytical ultracentrifugation of RAP, CR56, CR345 and their complexes.
| Species | Mr (kDa)* | Mr (kDa)† | sw,20‡ | f/f0§ | a/b¶ |
|---|---|---|---|---|---|
| RAP | 38±2 | 37.8 | 2.7±0.1 | 1.47±0.02 | 6.9±0.3 |
| CR56 | 7.8±0.3 | 8.9 | 1.49±0.02 | 1.46±0.04 | 5.5±0.5 |
| RAP–(CR56)2 | 53±3 | 55.6 | 3.79±0.02 | 1.38±0.04 | 4.7±0.6 |
| CR345 | 14.2±0.1 | 13.6 | 1.87±0.01 | 1.37±0.01 | 4.4±0.1 |
| RAP–(CR345)2 | 66±3 | 65 | 4.26±0.01 | 1.38±0.05 | 4.8±0.7 |
*Molecular mass calculated from sedimentation data.
†Molecular mass calculated from sequence and stoichiometry of 1:2 for RAP:(CR)x complexes.
†Temperature-corrected sedimentation coefficient at 20 °C in water.
§Frictional ratio.
¶Shape factor expressed as ratio of long to short axes (a/b) for a prolate ellipsoid.