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. Author manuscript; available in PMC: 2009 Jul 10.
Published in final edited form as: J Am Chem Soc. 2008 Jan 29;130(7):2195–2201. doi: 10.1021/ja074506u

Figure 2.

Figure 2

UV titration of: A. 4, B. 5, and C. 6 from pH 8.0 to 13.0 in the appropriate buffer (50 mM). The pKa’s were determined by plotting the UV absorbances of the two major peaks vs the pH, then fitting to a sigmoidal function (insets). The pKa’s of 4-6 are pH: 10 ± 0.15, 10.5 ± 0.15, and 8.2 ± 0.10, respectively.