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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Biochemistry. 2008 Aug 13;47(36):9661–9666. doi: 10.1021/bi800926x

Table 3.

Concentration of product (mM) generated by step-annealing the cryogenerated hydroperoxoferri-2B4/BHT complex.

Protein substrate complex [Fe-OOH]a [1]b [2]b [1] + [2]b
oxy-2B4/BHT (1:8)c 0.14 (3) 0.101 0.016 0.117
oxy-2B4/BHT (1:1)c 0.14 (3) 0.101 0.016 0.117
oxy-2B4(F429H)/BHT (1:8)c 0.16 (3) 0.007 nd 0.007
a

Hydroperoxoferri-2B4/BHT generated by 3 Mr radiation dose, as determined by EPR spin quantitations. Numbers in parentheses are uncertainties in last digit.

b

Product formation as measured by LC-MS/MS assay as described.

c

Total enzyme, 0.5 mM; initial concentration of oxy-2B4, 0.4 mM. Numbers in parentheses are the molar ratio, [oxy-2B4]/[BHT]