Table 2.

Fractions of unstable mutants (> 3 kT) from codons 96 to 289 before and after substitution.

	G	A	V	L	I	S	C	T	M	F	Y	W	H	K	R	D	E	N	Q	ALL
A	0/4		1/6			0/4		1/7		1/1						3/7				0.21
V	10/12	7/12		6/12	1/6				6/8	3/5		3/3				3/5	9/9			0.67
L			0/9		0/3	0/1			0/5	3/6			2/4		6/8				4/6	0.36
I			0/6	1/4		6/6		6/6	1/3	6/6	1/1					1/1		6/6		0.72
S	0/7	0/3		1/2	1/5		0/11	0/10		1/7	2/3			1/1	2/6			1/5		0.15
C	5/8	0/1				4/10		0/1		6/7	7/9	7/8	1/1		9/10			0/1		0.70
T		0/10			2/11	0/8			1/3					1/5	1/2			0/6		0.11
M			0/6	1/5	1/6			2/5						4/6	3/4					0.34
F	1/1		2/4	3/5	1/3	4/5	4/4				0/2									0.625
Y	1/1					6/6	6/6			0/5			7/7	1/1		7/7		6/6	1/1	0.875
W	1/1			0/1		1/1	0/1								1/1					0.60
H			0/1	2/5							2/7				4/6	1/6		1/4	2/6	0.34
K				0/1				0/3	0/3			1/1			1/5		1/4	0/4	1/3	0.17
R	3/15			0/12	1/3	2/13	0/9	2/4	0/2	1/1	1/2	6/6	3/10	1/4				1/2	3/8	0.26
D	0/7	0/3	1/8		0/1						2/8		2/7		1/1		0/7	0/8		0.12
E	2/11	0/9	0/10	0/2										5/11	1/1	1/10			1/8	0.16
N					1/8	0/9		0/7	0/1	1/1	3/6		2/6	0/6		1/8				0.15
Q				2/5									1/6	0/4	0/6		0/2			0.13
ALL	0.34	0.18	0.08	0.30	0.17	0.365	0.32	0.26	0.32	0.56	0.47	0.94	0.44	0.34	0.58	0.39	0.45	0.36	0.375

First column shows residues before mutation and first row shows residues after mutation. Each element means fraction of unstable mutations. For example, there are a total of 3 mutations from I to M, only one of them is unstable. There are a total of 6 mutations from Y to S. All of them are unstable. Elements with 100% unstable mutations are highlighted in bold. The last column shows the percentage of total unstable mutations from a given WT amino acid. For example, mutations from WT V residue result in 67% unstable core domain. The last row shows the percentage of total unstable mutations to a given amino acid. For example, 94% mutations to W are unstable.