Fig. 10.

Verification of subcellular localization of dehydration stress-regulated nuclear proteins. The cDNA of three dehydration-responsive nuclear proteins identified in rice were fused in-frame to the 5′-end of GFP and transiently expressed in Allium epidermal cells upon cell bombardment. After incubation for 24 h, Allium cells were visualized by using confocal microscopy. The phase-contrast micrograph of the Allium cells is shown in the left panel; the fluoresced image of the same is shown in the right panel. The GFP images represent localization of the following proteins fused to GFP: pCAM35S-GFP (A), α7 subunits of 20 S proteasome (A7P), A7P-GFP (B); β6 (B6S) subunits of 20 S proteasome, B6S-GFP (C); and putative WD repeat-containing protein, WD-GFP (D).