Effect of dehydration on water status, proline accumulation, cell membrane stability, photosynthetic pigments, and protein content. Four-week-old seedlings from eight different rice varieties were used for comparative estimation of RWC (A), proline accumulation (B), electrolyte leakage (C), MDA (D), chlorophyll a (E), chlorophyll b (F), carotenoid (G), and total protein (H) in a time-dependent manner under dehydration. All experiments were done in triplicates (n = 3), and average mean values were plotted against time.