Table I. Biochemical analysis of nuclear purity.

Nuclear purity was determined on the basis of marker enzyme activities specific for cytosol (alcohol dehydrogenase), microbody (catalase), mitochondria (fumarase hydratase), and plastid (glucose-6-phosphate dehydrogenase). Experiments were done in triplicates. CE, crude extract; N1, nuclear fraction without wash; N2, nuclear fraction after first wash; N, final purified nuclear fraction.

Fraction	Catalase		Alcohol dehydrogenase		Glucose-6-phosphate dehydrogenase		Fumarate hydratase
	Activity	% of CE	Activity	% of CE	Activity	% of CE	Activity	% of CE
	μmol/mg/min		μmol/mg/min		μmol/mg/min		μmol/mg/min
Crude extract	28.02 ± 0.58	100	3140 × 10−5 ± 0.00004	100	158 × 10−4 ± 0.00018	100	6920 × 10−3 ± 0.17	100
N1	5.01 ± 0.025	17.88	470 × 10−5 ± 0.00002	14.96	25.9 × 10−4 ± 0.00015	16.39	1090 × 10−3 ± 0.020	15.75
N2	1.08 ± 0.011	3.85	70 × 10−5 ± 0.00001	2.22	4.5 × 10−3 ± 0.00012	2.848	172.50 × 10−3 ± 0.0018	2.49
N	0.035 ± 0.004	0.12	3.3 × 10−5 ± 0.00003	0.1	0.36 × 10−4 ± 0.00014	0.22	12.58 × 10−3 ± 0.0019	0.18