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Targeted quantification and validation of potential biomarkers by pseudoMRM LC-MS/MS in a Q-TOF mass spectrometer. A, total ion chromatogram (TIC) produced when Q1 was set to transmit m/z 638 only in an LC-MS/MS analysis of urine. B, XICs of the indicated fragment ions produced upon CID of uromodulin peptide with m/z 638.03. C, XICs as in B with different mass window settings. D, plot of S/N as a function of XIC mass window.
