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Determination of the pKa of the nitronate form of nitrocyclopentane. Solutions of nitrocyclopentane (100 μM) were prepared in different buffer solutions, and the absorbance difference from that of the buffer that was obtained after the addition of ≈1 × 10−8 M OYE1 was plotted vs. the measured pH. The curve is a theoretical one for a pKa of 7.8.
