Figure 4.

The active site of OYE1 is displayed with nitrocyclohexene positioned such that the nitro group is H-bonded to His-191 and Asn-194 and the hydroxyl of Tyr-196 positioned to protonate carbon 1 of the cyclohexene ring. The coordinates of the residues were taken from the crystal structure of OYE1 with p-hydroxybenzaldehyde bound (Protein Databank ID code 1OYB). Nitrocyclohexene replaces p-hydroxybenzaldehyde in the active site. FMN, Flavin mononucleotide. The graphic was constructed by using the Insight II Molecular Modeling System from Biosym Technologies, San Diego.