Table 4.

pKa values for nitronates and for OYE1-catalyzed nitronate formation

Substrate	pKa	pKoye
Nitrocyclohexane	8.65	9.15
Nitrocyclopentane	7.80	9.10
Nitroethylbenzene	8.75	9.10

The pKa values are the thermodynamic constants obtained from experiments such as that in Fig 2. The pK_oye values are those obtained from the enzyme-catalyzed formation of the nitronates, as illustrated in Fig 3.