Fig. 2.

Saturation curves of acINV and saSST for sucrose. Production of glucose, fructose and kestose is determined at different sucrose concentrations with the recombinant enzyme preparations incubated at 28°C for 20 min. For AcINV sucrose concentrations up to 200 mM are used, whereas saSST was tested for up to 1 M of sucrose. AcINV is saturated at relatively low sucrose concentrations, whereas saSST shows increased product formation with higher sucrose concentrations