Table 4.
Statistical analysis of the effect of condition, the contrast of drought versus control for the sensitive (dS − cS), or the tolerant (dT − cT) cultivars, or the condition × tolerance group interaction on the expression of genes in the functional groups
| Bin | Bin Name | n | p-value (Wilcoxon rank sum test) | Number of significantly up- or down-regulated genes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| d − c | dS − cS | dT − cT | Inter | d − c down | d − c up | dS − cS down | dS − cS up | dT − cT down | dT − cT up | Inter down | Inter up | |||
| 1 | PS | 89 | 0.000 | 0.000 | 0.000 | 0.516 | 21 | 1 | 16 | 2 | 17 | 0 | 3 | 3 |
| 1.1 | PS.lightreaction | 55 | 0.000 | 0.000 | 0.000 | 0.937 | 18 | 0 | 11 | 1 | 14 | 0 | 1 | 1 |
| 1.1.1 | PS.lightreaction.photosystem II | 24 | 0.000 | 0.000 | 0.000 | 0.545 | 10 | 0 | 4 | 1 | 8 | 0 | 1 | 1 |
| 1.1.1.2 | PS.lightreaction.photosystem II.PSII polypeptide subunits | 19 | 0.000 | 0.000 | 0.000 | 0.821 | 8 | 0 | 4 | 0 | 6 | 0 | 0 | 1 |
| 1.1.2 | PS.lightreaction.photosystem I | 10 | 0.000 | 0.000 | 0.003 | 0.616 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
| 1.1.2.1 | PS.lightreaction.photosystem I.LHC-I | 3 | 0.163 | 0.096 | 0.518 | 0.270 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| 1.1.2.2 | PS.lightreaction.photosystem I.PSI polypeptide subunits | 7 | 0.004 | 0.013 | 0.006 | 0.958 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
| 1.1.4 | PS.lightreaction.ATP synthase | 3 | 0.226 | 0.098 | 0.592 | 0.870 | 2 | 0 | 2 | 0 | 2 | 0 | 0 | 0 |
| 1.1.5 | PS.lightreaction.other electron carrier (ox/red) | 9 | 0.153 | 0.037 | 0.940 | 0.435 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| 1.3.13 | PS.calvin cyle.rubisco interacting | 6 | 0.409 | 0.854 | 0.093 | 0.270 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| 2 | Major CHO metabolism | 59 | 0.158 | 0.700 | 0.052 | 0.493 | 0 | 5 | 0 | 4 | 1 | 5 | 0 | 2 |
| 2.2.2 | Major CHO metabolism.degradation.starch | 20 | 0.028 | 0.143 | 0.051 | 0.755 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 |
| 2.2.2.1 | Major CHO metabolism.degradation.starch.starch cleavage | 12 | 0.007 | 0.039 | 0.086 | 0.984 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
| 8 | TCA/org.transformation | 39 | 0.256 | 0.013 | 0.940 | 0.009 | 1 | 3 | 0 | 6 | 1 | 1 | 2 | 0 |
| 8.1 | TCA/org.transformation.TCA | 23 | 0.292 | 0.025 | 0.998 | 0.019 | 0 | 1 | 0 | 4 | 1 | 0 | 2 | 0 |
| 10.8 | Cell wall.pectin*esterases | 7 | 0.655 | 0.832 | 0.096 | 0.437 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
| 11 | Lipid metabolism | 184 | 0.156 | 0.011 | 0.787 | 0.006 | 2 | 14 | 4 | 24 | 4 | 3 | 3 | 0 |
| 11.6 | Lipid metabolism.lipid transfer proteins | 5 | 0.200 | 0.177 | 0.327 | 0.813 | 0 | 3 | 0 | 2 | 0 | 2 | 0 | 0 |
| 11.9 | Lipid metabolism.lipid degradation | 53 | 0.009 | 0.000 | 0.471 | 0.011 | 0 | 6 | 0 | 10 | 1 | 1 | 2 | 0 |
| 11.9.2 | Lipid metabolism.lipid degradation.lipases | 15 | 0.229 | 0.074 | 0.805 | 0.070 | 0 | 2 | 0 | 4 | 0 | 0 | 0 | 0 |
| 11.9.4 | Lipid metabolism.lipid degradation.beta-oxidation | 17 | 0.110 | 0.042 | 0.471 | 0.304 | 0 | 3 | 0 | 5 | 0 | 0 | 1 | 0 |
| 13 | Amino acid metabolism | 195 | 0.361 | 0.464 | 0.093 | 0.876 | 4 | 22 | 6 | 29 | 2 | 8 | 5 | 2 |
| 13.2 | Amino acid metabolism.degradation | 73 | 0.152 | 0.347 | 0.009 | 0.996 | 2 | 13 | 2 | 18 | 0 | 5 | 3 | 0 |
| 13.2.3 | Amino acid metabolism.degradation.aspartate family | 20 | 0.448 | 0.622 | 0.431 | 0.958 | 1 | 6 | 1 | 7 | 0 | 4 | 2 | 0 |
| 13.2.3.5 | Amino acid metabolism.degradation.aspartate family.lysine | 11 | 0.163 | 0.262 | 0.128 | 0.632 | 0 | 4 | 0 | 5 | 0 | 2 | 2 | 0 |
| 13.2.4 | Amino acid metabolism.degradation.branched chain group | 14 | 0.012 | 0.020 | 0.047 | 0.437 | 0 | 3 | 0 | 6 | 0 | 0 | 1 | 0 |
| 13.2.4.1 | Amino acid metabolism.degradation.branched-chain group.shared | 9 | 0.161 | 0.316 | 0.117 | 0.725 | 0 | 2 | 0 | 4 | 0 | 0 | 1 | 0 |
| 13.2.4.4 | Amino acid metabolism.degradation.branched-chain group.leucine | 4 | 0.160 | 0.056 | 0.499 | 0.330 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
| 13.2.6 | Amino acid metabolism.degradation.aromatic aa | 15 | 0.581 | 0.622 | 0.207 | 0.968 | 0 | 4 | 0 | 5 | 0 | 1 | 0 | 0 |
| 15 | Metal handling | 32 | 0.413 | 0.521 | 0.216 | 0.957 | 3 | 8 | 3 | 9 | 1 | 6 | 1 | 0 |
| 15.2 | Metal handling.binding, chelation and storage | 24 | 0.153 | 0.278 | 0.105 | 0.836 | 2 | 7 | 2 | 8 | 1 | 5 | 1 | 0 |
| 16 | Secondary metabolism | 138 | 0.428 | 0.037 | 0.790 | 0.024 | 4 | 11 | 9 | 9 | 2 | 5 | 2 | 1 |
| 16.1 | Secondary metabolism.isoprenoids | 43 | 0.047 | 0.011 | 0.484 | 0.444 | 1 | 3 | 4 | 3 | 1 | 0 | 2 | 0 |
| 16.8 | Secondary metabolism.flavonoids | 47 | 0.790 | 0.409 | 0.216 | 0.053 | 0 | 5 | 1 | 4 | 0 | 3 | 0 | 1 |
| 16.8.3 | Secondary metabolism.flavonoids.dihydroflavonols | 17 | 0.872 | 0.593 | 0.499 | 0.437 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
| 19 | Tetrapyrrole synthesis | 22 | 0.029 | 0.128 | 0.231 | 0.807 | 4 | 0 | 3 | 0 | 4 | 0 | 1 | 0 |
| 20 | Stress | 328 | 0.065 | 0.025 | 0.311 | 0.445 | 5 | 21 | 5 | 28 | 4 | 10 | 5 | 0 |
| 20.2 | Stress.abiotic | 172 | 0.066 | 0.004 | 0.499 | 0.135 | 3 | 14 | 3 | 18 | 3 | 8 | 2 | 0 |
| 21 | Redox.regulation | 96 | 0.883 | 0.502 | 0.916 | 0.588 | 3 | 11 | 4 | 15 | 2 | 4 | 3 | 0 |
| 26 | Misc | 403 | 0.000 | 0.000 | 0.000 | 0.601 | 10 | 35 | 13 | 46 | 7 | 17 | 12 | 4 |
| 26.9 | Misc.glutathione S transferases | 33 | 0.699 | 0.782 | 0.969 | 0.632 | 4 | 6 | 2 | 10 | 2 | 2 | 2 | 1 |
| 26.10 | Misc.cytochrome P450 | 56 | 0.006 | 0.177 | 0.009 | 0.843 | 2 | 6 | 3 | 5 | 2 | 4 | 4 | 2 |
| 26.12 | Misc.peroxidases | 18 | 0.428 | 0.099 | 0.499 | 0.034 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
| 26.21 | Misc.protease inhibitor/seed storage/lipid transfer protein (LTP) family protein | 7 | 0.089 | 0.050 | 0.759 | 0.179 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 |
| 27 | RNA | 1033 | 0.414 | 0.593 | 0.000 | 0.003 | 32 | 33 | 32 | 53 | 21 | 18 | 11 | 7 |
| 27.3 | RNA.regulation of transcription | 786 | 0.655 | 0.396 | 0.001 | 0.001 | 29 | 29 | 27 | 48 | 21 | 16 | 11 | 6 |
| 27.3.3 | RNA.regulation of transcription.AP2/EREBP, APETALA2/Ethylene-responsive element binding protein family | 20 | 0.443 | 0.177 | 0.998 | 0.018 | 0 | 3 | 0 | 4 | 0 | 1 | 2 | 0 |
| 27.3.24 | RNA.regulation of transcription.MADS box transcription factor family | 17 | 0.224 | 0.056 | 0.644 | 0.493 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| 27.3.99 | RNA.regulation of transcription.unclassified | 251 | 0.944 | 0.344 | 0.249 | 0.034 | 5 | 8 | 6 | 14 | 4 | 8 | 4 | 1 |
| 28 | DNA | 359 | 0.023 | 0.039 | 0.499 | 0.588 | 5 | 9 | 5 | 14 | 4 | 3 | 5 | 3 |
| 28.1 | DNA.synthesis/chromatin structure | 294 | 0.013 | 0.025 | 0.484 | 0.571 | 4 | 8 | 5 | 12 | 4 | 3 | 5 | 3 |
| 28.1.1 | DNA.synthesis/chromatin structure.retrotransposon/transposase | 181 | 0.023 | 0.095 | 0.327 | 0.858 | 1 | 3 | 2 | 5 | 1 | 0 | 3 | 3 |
| 29 | Protein | 1276 | 0.068 | 0.396 | 0.018 | 0.891 | 25 | 32 | 37 | 53 | 14 | 21 | 10 | 11 |
| 29.1 | Protein.aa activation | 56 | 0.042 | 0.004 | 0.787 | 0.036 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
| 29.2 | Protein.synthesis | 226 | 0.000 | 0.000 | 0.000 | 0.002 | 7 | 1 | 13 | 3 | 2 | 2 | 0 | 5 |
| 29.2.1 | Protein.synthesis.chloroplast/mito - plastid ribosomal protein | 37 | 0.359 | 0.000 | 0.327 | 0.000 | 4 | 0 | 6 | 0 | 1 | 2 | 0 | 5 |
| 29.2.1.1 | Protein.synthesis.chloroplast/mito - plastid ribosomal protein.plastid | 24 | 0.371 | 0.000 | 0.279 | 0.000 | 4 | 0 | 6 | 0 | 1 | 1 | 0 | 5 |
| 29.2.2 | Protein.synthesis.misc ribososomal protein | 116 | 0.000 | 0.000 | 0.000 | 0.445 | 3 | 1 | 7 | 2 | 0 | 0 | 0 | 0 |
| 29.5 | Protein.degradation | 520 | 0.066 | 0.000 | 0.945 | 0.009 | 7 | 18 | 13 | 33 | 7 | 11 | 4 | 5 |
| 29.5.3 | Protein.degradation.cysteine protease | 25 | 0.080 | 0.007 | 0.499 | 0.059 | 0 | 5 | 0 | 9 | 0 | 3 | 0 | 0 |
| 29.5.11 | Protein.degradation.ubiquitin | 305 | 0.056 | 0.000 | 0.973 | 0.006 | 1 | 7 | 2 | 15 | 3 | 4 | 1 | 2 |
| 29.5.11.4 | Protein.degradation.ubiquitin.E3 | 202 | 0.191 | 0.001 | 0.854 | 0.008 | 0 | 5 | 1 | 11 | 2 | 3 | 1 | 1 |
| 29.5.11.4.2 | Protein.degradation.ubiquitin.E3.RING | 100 | 0.416 | 0.071 | 0.897 | 0.220 | 0 | 3 | 1 | 5 | 1 | 2 | 0 | 0 |
| 29.5.11.4.3 | Protein.degradation.ubiquitin.E3.SCF | 85 | 0.361 | 0.093 | 0.940 | 0.437 | 0 | 1 | 0 | 5 | 1 | 0 | 1 | 1 |
| 29.6 | Protein.folding | 32 | 0.249 | 0.017 | 0.925 | 0.029 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| 30 | Signalling | 461 | 0.585 | 0.775 | 0.105 | 0.038 | 15 | 9 | 8 | 23 | 14 | 5 | 5 | 2 |
| 30.2.15 | Signalling.receptor kinases.thaumatin like | 6 | 0.681 | 0.323 | 0.905 | 0.516 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 30.3 | Signalling.calcium | 86 | 0.686 | 0.102 | 0.200 | 0.001 | 2 | 3 | 2 | 7 | 2 | 2 | 2 | 0 |
| 30.5 | Signalling.G-proteins | 68 | 0.156 | 0.093 | 0.499 | 0.588 | 0 | 2 | 1 | 6 | 1 | 2 | 1 | 2 |
| 31.4 | Cell. vesicle transport | 66 | 0.068 | 0.042 | 0.724 | 0.516 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 34 | Transport | 440 | 0.058 | 0.143 | 0.499 | 0.564 | 10 | 26 | 19 | 42 | 11 | 12 | 11 | 3 |
| 34.1 | Transport.p- and v-ATPases | 17 | 0.007 | 0.000 | 0.581 | 0.009 | 1 | 2 | 1 | 3 | 1 | 0 | 1 | 0 |
| 34.8 | Transport.metabolite transporters at the envelope membrane | 13 | 0.411 | 0.396 | 0.499 | 0.588 | 1 | 4 | 1 | 5 | 1 | 1 | 0 | 0 |
| 34.19 | Transport.Major Intrinsic Proteins | 13 | 0.191 | 0.036 | 0.797 | 0.516 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
| 34.19.2 | Transport.Major Intrinsic Proteins.TIP | 6 | 0.103 | 0.098 | 0.477 | 0.940 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
| 35 | Not assigned | 5324 | 0.700 | 0.025 | 0.231 | 0.000 | 88 | 143 | 118 | 201 | 68 | 76 | 54 | 56 |
| 35.1 | Not assigned.no ontology | 1839 | 0.300 | 0.062 | 0.998 | 0.539 | 28 | 57 | 35 | 76 | 17 | 32 | 25 | 22 |
| 35.1.40 | Not assigned.no ontology.glycine rich proteins | 11 | 0.028 | 0.021 | 0.173 | 0.773 | 0 | 3 | 0 | 4 | 0 | 2 | 0 | 1 |
| 35.2 | Not assigned.unknown | 3485 | 0.191 | 0.000 | 0.155 | 0.000 | 60 | 86 | 83 | 125 | 51 | 44 | 29 | 34 |
The probability of the Wilcoxon rank sum test (p(Wilcoxon)) indicates whether the average induction or interaction factor of all genes in a functional group is significantly higher (bold) or lower (italicized) than the average factor of all groups. Numbers of significantly up- or down-regulated genes are printed in bold, when a bin contains a significantly higher number of genes with significantly altered expression than the number averaged over all bins