Table 3.
RNAi system with transcription/translation machinery
| Reaction Number | Reaction | Rate Constant | Remarks |
| 1 | mRNA + siRNA -> gRNA | 0.008 hr-1 | |
| 2 | dsRNA -> 10*siRNA | 2.0 hr-1 | |
| 3 | mRNA -> dsRNA | 0.002 hr-1 | |
| 4 | siRNA -> ϕ | 2.0 hr-1 | |
| 5 | mRNA -> ϕ | 18 hr-1 | |
| 6 | gRNA -> ϕ | 2.8 hr-1 | |
| 7 | O + R -> O_R | 12.0 hr-1 | Regulatory molecule R binds to the operator region O to form the bound complex O_R |
| 8 | O_R -> O + R | 0.24 hr-1 | O_R dissociates into free R and O |
| 9 | P + RNAP -> P_RNAPC | 1.2 hr-1 | RNAP binds to promoter region P forming closed complex P_RNAPC |
| 10 | P_RNAPC -> P + RNAP | 0.06 hr-1 | P_RNAPC dissociates into free RNAP and P |
| 11 | P_RNAPC -> P_RNAPO | 48 hr-1 | Isomerization of closed to open complex (P_RNAPO) |
| 12 | P_RNAPO -> TrRNAP + mRNA + P | 54 hr-1 | RNAP clears promoter region and mRNA chain synthesis starts. TrRNAP denotes transcribing RNA polymerase. |
| 13 | TrRNAP -> RNAP | 4.8 hr-1 | RNAP completes transcription and is released from DNA. |
| 14 | mRNA + Ribosome -> RibRBS | 0.6 hr-1 | Ribosome binds to mRNA forming bound complex RibRBS |
| 15 | RibRBS -> mRNA+ Ribosome | 0.06 hr-1 | Ribosome dissociation from RibRBS |
| 16 | RibRBS -> EIRib + mRNA | 18 hr-1 | Ribosome EIRib initiates translation of mRNA chain |
| 17 | EIRib -> protein | 48 hr-1 | Protein synthesis by transcribing ribosome |
| 18 | protein -> ϕ | 0.18 hr-1 | Protein product degradation |