Figure 4.
The role of eve, runt, and odd in limiting slp and prd expression. Embryos either null mutant for eve (A) or double mutant for runt and eve (B–F) were stained (black) by in situ hybridization to RNA for either odd (A, B), slp (C, D), or prd (E, F), followed by either anti-En staining (A, B; brown) or anti-Eve staining (C–F; the embryos shown did not stain, indicating that they are mutant for eve). Note that odd expression, which is present in eve nulls (A), is absent from the trunk region in runt, eve double mutants (B), and that these same double mutants express both slp and prd throughout the trunk (C–F; prd has faded from most dorsal and lateral regions by late stage 8, seen in F).