TABLE 2.
Effect of oral Leu gavage on eIF4G·eIF4E association, eIF4E·4EBP1 association, eIF4E phosphorylation, and eIF4G phosphorylation in cardiac muscle following acute alcohol intoxication in rats1
| IP injection + gavage
|
||||
|---|---|---|---|---|
| Saline + water | Saline + Leu | Ethanol + water | Ethanol + Leu | |
| eIF4G·eIF4E association, eIF4G/eIF4E | 10 ± 1 | 17 ± 2 | 6 ± 1a | 13 ± 1 |
| eIF4G phosphorylation, phospho-eIF4G(Ser1108):total eIF4G | 19 ± 2 | 21 ± 2 | 10 ± 2b | 23 ± 3 |
| eIF4E·4EBP1 association, 4EBP1/eIF4E | 10 ± 1 | 8 ± 1 | 14 ± 0.7a | 10 ± 1 |
| eIF4E phosphorylation, phospho-eIF4E(Ser209):total eIF4G | 16 ± 3 | 9 ± 1 | 18 ± 3 | 10 ± 2 |
1
Values are means ± SEM; n = 6–13. Gavage with Leu affected all variables, P < 0.005. Letters indicate different from saline + water: aP < 0.05, bP < 0.001.