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Degradation of Δ4S by SpnUGL but not by BacillusUGL. The 10 mm substrate Δ4S or Δ6S incubated at 30 °C with 2 mg/ml BacillusUGL (A) or SpnUGL (B) or without enzyme (C) was subjected to TLC analysis. Lane 1, Δ4S; lane 2, Δ6S. The arrow indicates the position to which the developing solvent reached.
