TABLE 1.
Effects of reducing agents, monosaccharides, and metals on streptococcal UGL activity
| Chemical | Concentration | Relative activity |
||
|---|---|---|---|---|
| SagUGL | SpnUGL | SpyUGL | ||
| mm | % | |||
| None | 100 | 100 | 100 | |
| Reducing agents | ||||
| Dithiothreitol | 1 | 120 | 98 | 100 |
| Glutathione (reduced) | 1 | 140 | 130 | 94 |
| 2-Mercaptoethanol | 1 | 120 | 90 | 80 |
| Sugars | ||||
| d-GlcA | 5 | 1.6 | 3.3 | 2.2 |
| d-Glucose | 5 | 120 | 100 | 60 |
| d-Mannose | 5 | 100 | 100 | 78 |
| d-Galactose | 5 | 120 | 110 | 79 |
| l-Rhamnose | 5 | 110 | 110 | 65 |
| d-Glucosamine | 5 | 100 | 130 | 98 |
| d-Sucrose | 5 | 110 | 100 | 81 |
| d-Xylose | 5 | 110 | 110 | 67 |
| d-Galacturonic acid | 5 | 0.37 | 1.1 | NDa |
| 2-Deoxyglucose | 5 | 97 | 100 | 89 |
| l-Fucose | 5 | 95 | 100 | 50 |
| GalNAc | 5 | 90 | 97 | 95 |
| GlcNAc | 5 | 83 | 98 | 96 |
| Metals | ||||
| CoCl2 | 1 | 45 | ND | ND |
| CaCl2 | 1 | 92 | 101 | 97 |
| MnCl2 | 1 | 73 | 95 | 23 |
| MgCl2 | 1 | 91 | 101 | 110 |
| ZnCl2 | 1 | ND | ND | 6.1 |
| AlCl3 | 1 | 35 | 140 | ND |
| LiCl | 1 | 76 | 94 | 130 |
| NaCl | 1 | 78 | 96 | 130 |
| KCl | 1 | 78 | 97 | 130 |
a ND, not detected; 0.2% < of the control (none).