FIGURE 2.

Reduction of ARR19 expression by cAMP/LH treatments in MA-10 Leydig cells. A, MA-10 cells were treated with different doses of cAMP for 4 h prior to harvesting. B and C, MA-10 cells were treated with 300 μm cAMP and harvested at different time points. D, MA-10 cells were treated with different doses of LH for 2 h prior to harvesting. E, MA-10 cells were treated with 50 ng/ml LH and harvested at different time points. F, MA-10 cells transfected with (+) or without (−) GATA-1 expression plasmids were treated with or without 300 μm cAMP for 6 h prior to harvesting. A, B, D, E, and F, total cell extracts (50 μg of protein) were subjected to Western blot analysis for ARR19 and GATA-1 protein levels. DAX-1 was used as a positive control for the LH/cAMP response. C, total RNAs (20 μg) were subjected to Northern blot analysis for Arr19 and GATA-1 mRNA levels. G, coexpression of ARR19 and GATA-1 in the interstitial area of mouse testis. ARR19 expression was detected in a small number of cells in the interstitial compartment and haploid spermatids within seminiferous tubules (left column). GATA-1 expression was detected in some cells within the interstitial region (middle column). Overlapping of ARR19 and GATA-1 signals is shown in the right column. Arrowheads, cells that express ARR19 and GATA-1 within the interstitial region; Se, Sertoli cell; Sp, spermatid. 8Br-cAMP, 8-bromo-cyclic AMP.