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. 2009 Apr 27;284(27):18021–18032. doi: 10.1074/jbc.M900896200

FIGURE 4.

FIGURE 4.

Mapping and mutational analyses of GATA-1 binding sites in the ARR19 promoter. A, schematic representation of deletion mutants of the Arr19 promoter. B, HEK 293T cells were transiently transfected with each deletion mutant construct of the Arr19 promoter and GATA-1 expression plasmid along with (+) or without (−) the CBP expression construct. C, schematic representation of Arr19 promoter mutants. ΔCRE-ARR19-P1 is a mutated Arr19 promoter at the putative CRE with a 4-bp deletion. D, HEK 293T cells were transiently transfected with each promoter construct along with GATA-1 and CBP expression plasmid. E, Arr19 promoter constructs are schematically depicted with the indication of wild-type or mutated sequences (●) of GATA-1 binding motifs. MA-10 cells were transiently transfected with each promoter construct along with (+) or without (−) the GATA-1 expression plasmid. F, MA-10 cells were transiently transfected with each promoter construct and were treated with (+) or without (−) cAMP for 6 h prior to harvesting. Values represent the means ± S.E. of at least three independent experiments.