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. 2009 Apr 27;284(27):18021–18032. doi: 10.1074/jbc.M900896200

FIGURE 6.

FIGURE 6.

Association of GATA-1 and CBP protein with the GATA-1 motifs in the Arr19 promoter in MA-10 cells and in the testis. A, ChIP assays were conducted with MA-10 cells treated with 300 μm cAMP for 0, 3, and 6 h, and the chromatin was immunoprecipitated with anti-GATA-1 antibody and anti-CBP antibody. B, ChIP assays were conducted with the extract of 14-day-old mouse testis treated with hCG (10 IU, intraperitoneally) for 0, 3, and 6 h, and the chromatin was immunoprecipitated with anti-GATA-1 antibody and anti-CBP antibody. Immunoprecipitations with normal rabbit serum (Ctrl IgG) and without antibody (No Ab) or PCRs for β-actin DNA were employed as negative controls for nonspecific immunoprecipitations. PCRs were conducted with two sets of primer pairs in order to amplify the GATA-1 binding elements within the Arr19 promoter. Additional real time PCR was performed to quantify the relative amount of GATA-1 and CBP proteins associated with the Arr19 promoter under the treatment of hCG relative to the start point (0 h).