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Fractionation of PAI-1·(CR)x complexes by size exclusion chromatography. Elution profiles on a 47-ml Superdex 75 column of 9 μm cleaved PAI-1 preincubated with 0.5 eq of CR456 (solid line), with 3 eq of CR456 (●), or with 4 eq of CR56 (○). The elution positions of the individual protein components, run separately, together with those of bovine serum albumin (66 kDa) and the monomeric (29 kDa) and dimeric (58 kDa) forms of carbonic anhydrase are indicated at the top.
