TABLE 3.
Affinity of various PAI-1 species for CR fragments
Kd values are the average and spread of best fits to multiple independent determinations. ND, not determined.
| CR45 | CR345 | CR456 | CR567 | |
|---|---|---|---|---|
| Native | ||||
| Kd (nm) | ND | 16 ± 5 | 3 ± 1 | 38 ± 19 |
| ΔG0 (kJ mol−1) | −44.4 ± 0.8 | −48.5 ± 0.8 | −42.3 ± 1.3 | |
| Cleaved | ||||
| Kd (nm) | 18 ± 6 | 22 ± 4 | 2.7 ± 0.5 | 32 ± 10 |
| ΔG0 (kJ mol−1) | −44.1 ± 0.9 | −43.6 ± 0.5 | −48.8 ± 0.5 | −42.7 ± 0.8 |
| lmw-uPA | ||||
| Kd (nm) | 8 ± 3 | 8 ± 1 | ND | 5 ± 2 |
| ΔG0 (kJ mol−1) | −47.3 ± 1.0 | −46.1 ± 0.3 | −47.3 ± 1 | |
| Trypsin | ||||
| Kd (nm) | ND | 18 ± 2 | ND | 14 ± 1 |
| ΔG0 (kJ mol−1) | −44.1 ± 0.3 | −44.7 ± 0.2 | ||