FIGURE 2.

Lipolysis and re-esterification fluxes in hMADS adipocytes. A, release of lipolytic products NEFA and glycerol with/without Bay (10 μm) and/or FK (1 μm). B, [³H-9,10]OA and glycerol release in the presence of Triacsin C (10 μm) with/without Bay (10 μm) and/or FK (1 μm). C, correlation of [³H-9,10]OA release and re-esterification fluxes. Re-esterification was calculated as follows: [([³H-9,10]OA release with Triacsin C - [³H-9,10]OA release without Triacsin C)/[³H-9,10]OA release with Triascin C]. D, [³H-9,10]OA release and re-esterification fluxes of hMADS with/without Bay (10 μm) and/or FK (1 μm). All of the lipolytic and re-esterification fluxes were measured over 3 h. The data are presented as the means ± S.E. (n = 4–5). For one-way ANOVA, * or §, p < 0.05, significantly different from own basal; for two-way ANOVA, #, p < 0.05, significantly different from basal condition.