FIGURE 5.

TGase is important for the EGF-stimulated invasive activity in HeLa cells. A and B, graphs representing the invasive activities of HeLa cells exposed to various culturing conditions, as assessed using the Matrigel Transwell assay system. In A, the cells were incubated without or with EGF ± MDC for 1 day before being scored for invasiveness. In B, HeLa cells expressing control-RNAi, TGase-RNAi 1, or TGase-RNAi 2 were incubated without or with EGF for 1 day before being scored for invasiveness. These assays were performed at least three times, and the results were averaged together and graphed. The error bars indicate ±S.D.