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A, final purification step (anion exchange chromatography) of processing enzyme activity from C. albipes. Elution position of activity with phalloidin peptide precursor and activity with Z-Gly-Pro-pNA is indicated. B, SDS-PAGE of purified processing enzyme activity. Lane 1, size markers (kDa). Lane 2, 0.1 μg of purified cleavage enzyme. Lane 3, 0.5 μg of enzyme. mAU, milliabsorbance units.
