FIGURE 1.

PALB2 controls focal accumulation of BRCA2 and RAD51 at DNA damage site. A, cells were irradiated with 10 Gy of ionizing radiation and recovered for 5 h. Immunofluorescence staining was performed using antibodies as indicated. C, PALB2-deficient EUFA1341 cells and its derivative (EUFA1341+PALB2) were treated with 10 Gy of ionizing radiation and recovered for 5 h or 1 mm MMC for 24 h. Cell lysates were separated by fractionation to retrieve both nuclear and chromatin-associated proteins. The presence of PALB2, BRCA2, and RAD51 was examined by Western blot analyses as indicated. B, RAD51 foci formation was examined in indicated cell lines using anti-RAD51 antibody. DAPI, 4′,6-diamidino-2-phenylindole.