FIGURE 1.
Affinities of Bak for different Bcl-2 variants, Mcl-1, and Bcl-xLin vitro. A, different Bcl-2 variants used in this study, with GenBankTM accession numbers and original citations. Amino acids that differ among the variants are shown. Our sequencing demonstrated that Jurkat, CEM, and Molt3 all express variant 2. B, surface plasmon resonance (relative units) observed when immobilized BakΔTM was exposed to different Bcl-2 (variant 1) concentrations. C, response of the same chip to different concentrations of Mcl-1. D, using the same chip as in B, responses of Bcl-2 (variant 1) and Bcl-xL at a saturating concentration of 2400 nm. E, based on the surface plasmon resonance assay, the affinities of Bak protein (B and C) and Bak BH3 peptide (Fig. S1, A–C) for different anti-apoptotic Bcl-2 family proteins were determined. Results are mean ± S.D. of three independent experiments using different chips and different protein batches. †, KD >1000 nm. GB, GenBankTM.