FIGURE 6.

Binding of WRKY to the cwp1 and cwp2 promoters. A, WRKY binding site was required for the binding. Electrophoretic mobility shift assays were performed using purified WRKY and a ³²P-end-lableled oligonucleotide probe from the 5′-flanking region of the cwp1 and cwp2 genes as described. The arrowhead indicates the shifted complex. The putative GTCA or ATCA core sequence in W box is framed and underlined, respectively. The mutated sequence is shown in underlined lowercase letters. + and − represent DNA binding and no binding, respectively. B, mutation analysis of the WRKY binding site in the cwp2 promoter region. Data are presented as in Fig. 5D, except that the luciferase gene is flanked by the 5′-flanking region of the cwp2 gene. The transcription start site is indicated by an arrowhead (6).