FIGURE 3.

Probing a glycan array with a tetrameric complex of Alexa 488-labeled streptavidin with biotin-tagged CRD from prolectin. The protein concentration was 0.45 mg/ml. Glycans that are bound by the probe are colored based on the terminal residues present: red for fucose, green for mannose, and blue for N-acetylglucosamine. Structures of the ligands that give the strongest signals are indicated using symbol nomenclature. A complete list of all the glycans on the array is provided in supplemental Table S1.