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Trafficking of prolectin expressed in fibroblasts. A, pH dependence of ligand binding. Binding of 125I-Man-BSA to immobilized extracellular domain of prolectin was assayed at various pHs in the presence of 2 mm Ca2+. B, uptake and degradation of 125I-Man-BSA by fibroblasts expressing prolectin or DC-SIGN. Degradation was measured as acid-soluble fragments appearing in the medium.
