FIGURE 7.

MN1 knock-out calvarial cells have reduced expression of osteoblastic genes in vitro and in vivo. A, WT and MN1 KO osteoblasts were cultured under osteogenic conditions for the indicated times. The expression of BSP2, DMP1, Runx2, osterix, and 18 S RNA were determined by TaqMan real-time reverse transcription-PCR approaches using 18 S RNA as an internal control. B, total RNA was extracted from WT and MN1 KO E18.5 calvaria. The expression of MN1, Runx2, osterix, BSP2, alkaline phosphatase (ALP), osteocalcin (OC), and cyclophilin B (CypB) was examined by Northern blot analysis. Cyclophilin B expression was used as a loading control.