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. 2009 May 11;284(27):18210–18217. doi: 10.1074/jbc.M109.000034

FIGURE 3.

FIGURE 3.

Inhibition of SirT1 stimulates cell proliferation. a, H1299 cells were transiently transfected with cyclin D3 promoter-luciferase, E2F1, SirT1, and SirT1-H363A mutant. Promoter activity was measured by luciferase assay and normalized to cotransfected CMV-lacZ level. Error bars represent mean ± S.D. (n = 4). b, 293T cells were transiently transfected with E2F1, CREB-binding protein, and SirT1. E2F1 was immunoprecipitated and probed with anti-Ac-K117 antibody by Western blot. The membrane was reprobed for E2F1 and SirT1 expression levels. c, wild-type and E2F1−/− MEFs were cultured for 18 h in 1% serum and 2 μm EX-527. DNA replication was measured by [3H]thymidine incorporation assay. Error bars represent mean ± S.D. (n = 8). For wild-type MEF cells, p = 0.002 indicates significant difference in proliferation after treatment. For E2F1−/− MEFs, p = 0.7 (>0.05) indicates no effect by EX-527 treatment.