FIGURE 2.

RUNX2 transcriptional activity in response to glucose. A and B, HBME cells were transfected with the reporter 6XOSE2.luciferase vector and TK.renilla vector (to normalize for transfection efficiency) prior to starvation and subsequent treatment with 5 mm glucose for the indicated time (A) or glucose dose (B) for 6 h. Firefly luciferase activity was determined in cell lysates and expressed relative to Renilla luciferase. Asterisk indicates the following p values: A, comparing transcriptional activation at 8 h versus t = 0 (p < 0.05); B, at 5 mm versus 25 mm glucose (p < 0.01).