FIGURE 8.

Hyperglycemia-induced oxidative stress inhibits RUNX2 activity and wound healing. A, EC were serum- and glucose-starved prior to incubation with glucose and the antioxidant NAC (10 mm) for 4 h. Nuclear extracts were analyzed for RUNX2 DNA binding using EMSA. B, EC wound healing assays were performed as described with cells treated for 24 h with 25 mm glucose in the presence or absence of 10 mm NAC. C, percent wound healing was calculated as described previously and is representative of n = 5 measurements per treatment condition. *, p < 0.01 for 25 mm versus 25 mm + NAC.