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Cleavage of the fluorescein-labeled peptide substrate LF15F by LF E687D (A) and LF H690A (B) mutant enzymes examined by stopped flow. Fluorescence increase measured during the enzyme cleavage was used as the readout. The substrate concentration was 5 μm; enzyme concentrations are indicated to the right of the panels. The calculated kinetic parameters are shown in Table 4.
