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ProNGFhis expressed in mammalian cells induces MAPK phosphorylation. PC12 cells were treated as described under “Experimental Procedures” with proNGFhis or WT-NGFhis expressed in HEK-293 cells. A, Western blotting for phosphorylated MAPK and total MAPK. B, proNGFhis remains unprocessed after the MAPK assay.
