FIGURE 9.

ProNGF induces cell death in unprimed PC12 cells after serum removal. Unprimed PC12 cells cultured as described under “Experimental Procedures” were treated with 0.4 nm of either WT-NGF (A) or proNGFhis (B) in serum-free medium and stained with TUJ-1 antibody and Hoechst after 48 h treatment. There were more dead cells in cultures treated with proNGFhis (B) compared with WT-NGF (A) and medium alone (C). ProNGFhis induced more apoptosis compared with NGF and medium alone (D). Yellow arrows show representative live cells, and white arrows show representative dead cells. *, p < 0.05, one way analysis of variance followed by post hoc Tukey's test. Error bars represent S.E. for n = 3 per group.