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Purification of histidine-tagged Pol II from yeast. (A) Outline of purification scheme for 6-histidine-tagged Pol II. (B) Analysis of proteins in the Pol II preparation by SDS-PAGE. The subunits of Pol II (Rpb1–12) are indicated. (C) Western blot with anti-yTBP antibody. Twenty micrograms (about 15 pmol) purified Pol II was loaded onto the gel. The indicated amounts of purified recombinant yTBP were loaded onto the gel as quantitation standards.
