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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Trans R Soc Trop Med Hyg. 2008 Dec;102(Suppl 1):S152–S162. doi: 10.1016/S0035-9203(08)70033-6

Figure 1.

Figure 1

Comparison of growth and bioluminescence of ΔglmU and ΔsecA strains carrying the GlmU-depletion responsive promoter from PA1494 fused to P. luminescens luxCDABE. Bioluminescent reporter strains MDM1142 (ΔglmU) and MDM1169 (ΔsecA), both containing the PA1494-luxCDABE element, were grown overnight in LBGS containing 0.05 mM IPTG (■)(control culture), 0.025 mM ITPG (△), or 0.0125 mM IPTG (◇), then subcultured into LBGS without IPTG, except for the control, which was maintained at 0.05 mM IPTG. Growth (OD600) was followed for 7.5 h for (A) MDM1142 (solid lines) and (B) MDM1169 (dashed lines). (C) Cells (200 μl) from each culture were added to 96-well opaque white microplates after 3 h, and relative light units (RLU) were measured for an additional 4.5 h.