TABLE 1.
Strains and Plasmids
| Straina or Plasmid | Relevant Genotypeb | Source |
|---|---|---|
| P. aeruginosa strains: | ||
| PAO-LAC | Wild type strain PAO1 with lacIQ lacZ′ inserted at attB in chromosome via mini-CTX-lacM15 | (Hoang et al., 2000) |
| Complemented P. aeruginosa deletions: | ||
| MDM271 | PAO-LAC Δ gyrA::TcR / pUCP24GW-lacPO-Pae-gyrA | This study |
| MDM275 | PAO-LAC Δ coaD::TcR / pUCP24GW-lacPO-Bpseu-coaD | This study |
| MDM339 | PAO-LAC Δ ftsQ::TcR / pUCP24GW-lacPOBpseu-ftsQ | This study |
| MDM349 | PAO-LAC Δ gyrA::TcR / pUCP24GW-lacPO-Bpseu-gyrA | This study |
| MDM359 | PAO-LAC Δ lpxA::TcR / pUCP24GW-lacPO-Bpseu-lpxA | This study |
| MDM363 | PAO-LAC Δ mesJ::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-mesJ | This study |
| MDM498 | PAO-LAC Δ efp::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-efp | This study |
| MDM502 | PAO-LAC Δ glmU::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-glmU | This study |
| MDM504 | PAO-LAC Δ ligA::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-ligA | This study |
| MDM510 | PAO-LAC Δ secA::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| MDM882 | PAO-LAC Δ folA::TcR / pUCP24GW-lacPO-Bpseu-folA | This study |
| MDM949 | PAO-LAC Δ ipk::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-ipk | This study |
| MDM366 | PAO-LAC Δ dnaA::TcR / pUCP24GW-lacPO-Bpseu-dnaA | This study |
| MDM924 | PAO-LAC Δ ftsA::TcR / pUCP24GW-lacIQ-lacPO-Bpseu-ftsA | This study |
| Reporter strains and complemented P. aeruginosa deletion reporter strains: | ||
| MDM977 | PAO-LAC Δ gyrA::TcR::pGSV3-‘PA0614’-luxCDABE / pUCP24GW-lacPO-Pae-gyrA | This study |
| MDM981 | PAO-LAC Δ gyrA::TcR::pGSV3-‘PA0614’-luxCDABE / pUCP24GW-lacPO-Bpseu-gyrA | This study |
| MDM1141 | PAO-LAC Δ coaD::TcR::pGSV3-‘PA4607’-luxCDABE / pUCP24GW-lacPO-Bpseu-coaD | This study |
| MDM1142 | PAO-LAC Δ glmU::TcR::pGSV3-‘PA1494’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-glmU | This study |
| MDM1143 | PAO-LAC Δ secA::TcR::pGSV3-‘PA2403’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| MDM1144 | PAO-LAC Δ secA::TcR::pGSV3-‘PA2408’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| MDM1145 | PAO-LAC Δ secA::TcR::pGSV3-‘PA1365’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| MDM1164 | PAO-LAC Δ glmU::TcR::pGSV3-‘PA1471’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-glmU | This study |
| MDM1166 | PAO-LAC Δ glmU::TcR::pGSV3-‘PA2358’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-glmU | This study |
| MDM1167 | PAO-LAC Δ secA::TcR::pGSV3-‘PA2404’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| MDM1168 | PAO-LAC Δ coaD::TcR::pGSV3-‘PA2030’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-coaD | This study |
| MDM1169 | PAO-LAC Δ secA::TcR::pGSV3-‘PA1494’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| MDM1170 | PAO-LAC Δ glmU::TcR::pGSV3-‘PA1365’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-glmU | This study |
| MDM1184 | PAO-LAC Δ secA::TcR::pGSV3-‘PA1471’-luxCDABE / pUCP24GW-lacIQ-lacPO-Bpseu-secA | This study |
| Plasmids: | ||
| pEX18ApGW | pEX18Ap modified with Invitrogen Gateway Vector Conversion kit | (Choi and Schweizer, 2005) |
| pUCP24GW | pUCP24 modified with Invitrogen Gateway Vector Conversion Kit | (Moir et al., 2007) |
| pUCP24GW-lacIQ | pUCP24GW modified by addition of lacIQ at BspHI site | (Moir et al., 2007) |
| pGSV3-lux-SpR | pGSV3-lux modified by replacement of GmR with SpR element at SacI sites | This study |
a
Commonly used E. coli hosts and cloning vectors are described and referenced in Materials and Methods
b
ApR, SpR, GmR, and TcR: ampicillin, spectinomycin, gentamicin, and tetracycline resistance, respectively.