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. Author manuscript; available in PMC: 2010 May 19.
Published in final edited form as: Biochemistry. 2009 May 19;48(19):4159–4169. doi: 10.1021/bi900224c

Figure 10. ESI/Q-TOF mass spectrometry of NO2-LA adducts with GSTP1a.

Figure 10

Recombinant GSTP1a-1a samples incubated without (panel A) and with (panel B) a 20-fold molar excess of NO2-LA were prepared as described in Figure 9 and processed for mass spectrometry according to Experimental Procedures. Raw ion data were transformed by maximum entropy calculations to yield masses corresponding to unmodified GSTP1a monomers (23,223 [-met] and 23,354 [+met]) and to GSTP1a monomers adducted with one NO2-LA (23,548 [-met] and 23,679 [+met]) or two NO2-LA (23,873 [-met] and 24,004 [+met]) molecules.